Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study.

نویسندگان

  • Fatma Helvacioglu
  • Ersin Kandemir
  • Busra Karabacak
  • Idil Karatas
  • Ahmet Pecen
  • Ipek Ercan
  • Tugce Sencelikel
  • Attila Dagdeviren
چکیده

AIM Creatine is an endogenous molecule synthesized in the liver, kidney and pancreas from glycine and arginine and is important for mitochondrial metabolism. It is widely used as a supplement for improving muscle mass and function for many years. As it is expected to prevent apoptosis and diminish oxidative stress, it is also studied in a number of neurodegenerative diseases for its beneficial effect in recent years. We studied the effect of creatine on the peripheral nerve injury in an experimental rat crush injury model to obtain ultrastructural evidence. MATERIAL AND METHODS Animals were randomly divided into 3 groups having 5 animals in each group. Group 1 was the control group, Group 2 the trauma group and Group 3 the trauma+creatine group. The first group served as sham control. In group 2 and group 3, sciatic nerves of the rats received crush injury using aneurysm clips. In group 3, daily 2 g/kg creatine monohydrate was administered via gavage after the trauma. Nerve samples were obtained at the 28th day after trauma for light and electron microscopic evaluation. RESULTS Our comparative analysis results suggest a possible positive effect of creatine supplement on peripheral nerve regeneration as statistical analysis revealed significant differences between group 2 and group 3. Though our finding does not represent a miracle of regenerative support, beneficial usage of creatine is documented in the present study. CONCLUSION Creatine supplement helps to diminish the harmful effects of peripheral nerve crush injury which is also supported by electron microscopy findings.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Ultrastructural Changes in Spinal Motoneurons and Locomotor Functional Study after Sciatic Nerve Repair in Conduit Tube

Objective(s) Motor deficit and neuron degeneration is seen after nerve transection. The aim of this study is to determine whether a poled polyvinelidene fluoride (PVDF) tube with other supportive strategies can protect the neuronal morphology and motor function after sciatic nerve transaction in rats. Materials and Methods After transection of the left sciatic nerve in 60 male Wistar rats (2...

متن کامل

P49: The Evaluation of Aqueous Extract of Glycyrrhiza Glabra on Nerve Recovery in the Rat after Sciatic Nerve Injury

Peripheral nerve injury requires a long recovery period, and recovery, once attained, usually is incomplete. Inflammatory procedures may inhibit functional recovery after nerve injury and produce cell death in both the central nervous system and the peripheral nervous system. Since the glycyrrhiza glabra extract has anti-inflammatory effects, it could reduce the severity of injury. The aim of t...

متن کامل

Age-Dependent Regeneration by Using Electromyographical Study Foliowing Sciatic Nerve Injury in Rat

Purpose: There are extensive evidences that show axonal processes of the nervous system (peripheral and/or central) may be degenerated after nerve injuries. Axonal regeneration is relation to various factors. In this investigation we decided to evaluate the effects of nerve regeneration age-dependent on injured rat sciatic nerv. Materials and Methods: For this study, the right sciatic nerve of...

متن کامل

Immune cell distribution and immunoglobulin levels change following sciatic nerve injury in a rat model

Objective(s): To investigate the systemic and local immune status of two surgical rat models of sciatic nerve injury, a crushed sciatic nerve, and a sciatic nerve transection Materials and Methods:Twenty-four adult male Sprague-Dawley rats were randomly divided into three groups: sham-operation (control group), sciatic nerve crush, and sciatic nerve transaction. Sciatic nerve surgery was perfor...

متن کامل

Histopathological effects of intramuscular metamizole sodium on rat sciatic nerve

Objective(s): We investigated the histopathological effects of metamizole sodium (MS) on the sciatic nerve.  Materials and Methods: This study was performed using 48 adult male Wistar albino rats. Ten groups were constituted with 6 rats in each group. MS injection into the sciatic nerve (group 1), MS injection into the muscle [group 3 (50 mg/kg, 0.4 ml) and group 5 (50 mg/kg, 0.8 ml)], MS injec...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Turkish neurosurgery

دوره 28 1  شماره 

صفحات  -

تاریخ انتشار 2018